■ 基本信息
啟動子: | 無 |
復制子: | pVS1 oriV，pUC ori |
終止子: | NOS terminator |
質粒大小: | 10658bp |
原核抗性: | Kan |
真核抗性: | Hyg |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
■ 質粒屬性
質粒宿主: | 植物 |
質粒用途: | 蛋白表達 |
片段類型: |
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片段物種: |
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原核抗性: | Kan |
真核抗性: | Hyg
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熒光標記: |
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■ 質粒簡介
pCAMBIA1391是一個植物啟動子檢測報告載體，可通過MCS克隆進需要研究的啟動子。
The pCambia vector backbone is derived from the pPZP vectors. The pCambia1391 vector offers:
High copy number in E.coli for high DNA yields ? pVS1 replicon for high stability in Agrobacterium
Small size ? Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with kanamycin
Plant selection with hygromycin B
Simple means to construct translational fusions to gusA reporter genes
Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV35S promoter and terminated by the CaMV35S polyA signal. Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins. Designed to utilize gusA as a true reporter of gene expression by fusion construction this vector contains a promoterless, non-intron gusA gene that retains the initiation codon of the NcoI site. This permits simple construction of carboxy-terminus protein fusions to gusA.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK1712 pCAMBIA1391植物表達質粒.txt
