■ 基本信息
別名: | Z32698.1 |
啟動(dòng)子: | gpdA |
復(fù)制子: | pUC |
終止子: | TRPC |
質(zhì)粒分類: | 其他宿主載體；絲狀真菌表達(dá)質(zhì)粒 |
質(zhì)粒大小: | 6756bp |
原核抗性: | Amp |
篩選標(biāo)記: | Hyg |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 絲狀真菌 |
培養(yǎng)條件: | 參考相關(guān)文獻(xiàn) |
誘導(dǎo)方式: | 無需誘導(dǎo) |
5'測序引物: | 根據(jù)全序列設(shè)計(jì) |
3'測序引物: | 根據(jù)全序列設(shè)計(jì) |
備注: | NCBI號(hào)：Z32698.1 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 絲狀真菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | Hyg |
熒光標(biāo)記: |
|
■ 質(zhì)粒簡介
參考文獻(xiàn)
1、Effect of de-phosphorylation of linearized pAN7-1 and of addition of restriction enzyme on plasmid integration in Penicillium paxilli.
2、Transformation of sapstaining fungi with hygromycin B resistance plasmids pAN7-1 and pCB1004.
3、Padro de integrao de pAN7-1 em mutantes de Magnaporthe grisea com patogenicidade alterada em arroz.
4、Integra??o de pAN7-1 no Genoma de Magnaporthe grisea Mediada por Enzima de Restri??o.
5、Primary structure of the trpC gene from Aspergillus nidulans.
6、Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli.
7、Functional elements in the promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK1061pAN7-1真菌表達(dá)質(zhì)粒.txt
注：原作者未公布原始序列，官網(wǎng)充列僅供參考，可聯(lián)系負(fù)責(zé)人索要測序結(jié)果。
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
