■基本信息
啟動子: | TRP1 ,T7,T3 |
復(fù)制子: | pUC |
質(zhì)粒分類: | 酵母系列，酵母單雜交載體 |
質(zhì)粒大小: | 7207bp |
原核抗性: | Kan |
真核抗性: | TRP1 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 酵母細胞 |
5'測序引物: | M13F:TGTAAAACGACGGCCAGT |
3'測序引物: | 根據(jù)序列設(shè)計引物 |
■質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 雜交 |
片段類型: |
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片段物種: |
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原核抗性: | Kan |
真核抗性: | TRP1
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熒光標記: |
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■質(zhì)粒簡介
pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker? One-Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene,located downstream of a multiple cloning site (MCS) and the minimal promoter of the HIS3 locus(PminHIS3). Cis-acting DNA sequences, or DNA target elements, can be inserted into the MCS andused as baits to screen GAL4 AD/cDNA fusion libraries for proteins that interact with the targetsequence. A protein-DNA (or one-hybrid) interaction can be detected by performing the assay in ayeast strain such as Y187 that is auxotrophic for histidine. Positive one-hybrid interactions driveexpression of the HIS3 reporter gene, which enables the host cell to grow on histidine-deficientmedia.
■質(zhì)粒圖譜
■質(zhì)粒序列
質(zhì)粒序列請下載：
ZK971pHIS2酵母單雜交質(zhì)粒.txt
