■ 基本信息
啟動(dòng)子: | TRP1，T3,T7 |
復(fù)制子: | pUC |
質(zhì)粒分類(lèi): | 酵母系列，畢赤酵母表達(dá)載體 |
質(zhì)粒大小: | 4783bp |
原核抗性: | Amp |
真核抗性: | TRP1 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 酵母細(xì)胞 |
誘導(dǎo)方式: | 半乳糖誘導(dǎo) |
5'測(cè)序引物: | M13R:CAGGAAACAGCTATGACC |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類(lèi)型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | TRP1
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
YCtype centromere vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ) peptide. pRSS56, constructed by ligating a PvuI fragment (bp 4982412) of pBluescript KS+ to a PvuI fragment (bp 2850 730) of pBS(+), contains the KS MCS from pBluescript KS(+) and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A genomic HindII/PstI fragment (1.002 kb) containing the TRP1 gene was inserted into the NdeI site and a cassette containing CEN6 and the ARS associated with histone 4 (ARSH4) was inserted into the AatII site of pRSS56. All ends were blunted. An EcoRI site in the TRP1containing fragment (external to the coding sequence) was destroyed. The order of the major features in this plasmid is: TRP1f1 ori (NaeI) T7 promoter lacZ/MCS T3 promoter pMB1 ori bla CEN6 ARSH4.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK956pRS314釀酒酵母質(zhì)粒.txt
