■ 基本信息
啟動(dòng)子: | URA3 |
復(fù)制子: | 2μ |
質(zhì)粒分類: | 酵母系列，釀酒酵母表達(dá)載體 |
質(zhì)粒大小: | 5181bp |
原核抗性: | Amp |
真核抗性: | URA3 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 酵母細(xì)胞 |
5'測(cè)序引物: | M13F:TGTAAAACGACGGCCAGT |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | URA3
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
Restriction digests of the clone give the following sizes (kb): BamHI--5.3; XbaI--5.3; PstI--5.3; HindIII--5.3.This is a Escherichia coli/yeast episomal shuttle vector with multiple restriction sites for cloning.Constructed by filling in and blunt-end ligating pUC18 linearized at NdeI, a 1.4 kb RsaI fragment of the 2 micron circle, and a 1.1 kb HindIII/SmaI fragment containing URA3. A XbaI site in 2u and a PstI site in URA3 were removed.The yeast genomicsequence is in nt 286-1383 (where the A of the ATG of lacZ' is number 1), the 2 micron sequence goes from nt 1384-2768, and pUC18 includes nt 2769-285.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK954pYEp352釀酒酵母質(zhì)粒.txt
