■ 基本信息
啟動(dòng)子: | T7 |
復(fù)制子: | ori |
終止子: | T1,T2 |
質(zhì)粒分類: | 植物系列，gateway載體 |
質(zhì)粒大小: | 4291bp |
質(zhì)粒標(biāo)簽: | ccdB |
原核抗性: | Zeo |
克隆菌株: | DB3.1 |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 植物細(xì)胞 |
5'測(cè)序引物: | M13F:TGTAAAACGACGGCCAGT |
3'測(cè)序引物: | M13R:CAGGAAACAGCTATGACC |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 大腸桿菌 |
質(zhì)粒用途: | 基因模板 |
片段類型: | ORF,GateWay |
片段物種: | 空載體 |
原核抗性: | Zeo |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
pDONRvectors are Gateway?-adapted vectors designed to generate attL-flanked entry clones containing your gene of interest following recombination with an attB expression clone or an attB PCR product. Once you have created an entry clone, your gene of interest may then be easily shuttled into a large selection of expression vectors using the Gateway? LR recombination reaction.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK874pDONR221Zeo大腸克隆質(zhì)粒.txt
