■ 基本信息
啟動子: | U6 |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 病毒系列，慢病毒克隆載體 |
質(zhì)粒大小: | 7757bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無須誘導(dǎo)，瞬時表達(dá) |
5'測序引物:
| U6:ATGGACTATCATATGCTTACCGTA |
3'測序引物: | 根據(jù)序列設(shè)計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞,慢病毒 |
質(zhì)粒用途: | 基因沉默 |
片段類型: | shRNA
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | Puro
|
熒光標(biāo)記: |
|
■ 質(zhì)粒簡介
pLVX-shRNA1 is an HIV-1-based, lentiviral expression vector designed to express a small hair-pin RNA (shRNA) for RNA interference (RNAi) studies. Expression of your shRNA is drivenby the RNA Pol III-dependent, human U6 promoter (PU6), located just upstream of the MCS. pLVX-shRNA1 can either be used as a plasmid expression vector and transfected into cells, or it can be packaged into viral particles and transduced into cells. Lentiviral particles derived from the vector allow the expression of shRNAs in virtually any cell type, including primary cells.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK816pLVX-shRNA1慢病毒干擾質(zhì)粒.txt
