■ 基本信息
別名: | pCDH-EF1α-MCS-T2A-Puro |
啟動(dòng)子: | EF1α |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 病毒系列，慢病毒克隆載體 |
質(zhì)粒大小: | 7087bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞,慢病毒 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
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片段物種: |
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原核抗性: | Amp |
真核抗性: | Puro
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
Most cell types (split and non split cells); primary cell differentiation pCDH-EF1-MCS-T2A-Puro lentiviral expression vector of lentiviral vector is based on HIV for cDNA; cloning and expression; transfection efficiency of cells, to establish a stable cell line; single promoter driven by EF1a promoter and gene transcription and resistance genes for bicistron; between the two with a T2A peptide to shear on the translation level on the self, to ensure that the same genes and resistance gene translation level.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK781pCDH-EF1-MCS-T2A-Puro慢病毒表達(dá)質(zhì)粒.txt
