■ 基本信息
別名: | Plasmid #16405 |
啟動子: | CMV |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒大小: | 9237bp |
原核抗性: | Kan |
真核抗性: | EGFP |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
誘導(dǎo)方式: | 無須誘導(dǎo)，瞬時表達 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞,腺病毒 |
質(zhì)粒用途: | 蛋白表達 |
片段類型: |
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片段物種: |
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原核抗性: | Kan |
真核抗性: |
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熒光標(biāo)記: | 綠色
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■ 質(zhì)粒簡介
PAdTrack-CMV transgenic adenovirus vector to cell expression vector with CMV promoter and EGFP gene expression of green fluorescent protein tracking results, and use common pAdeasy1 vector maximum can be inserted into the 5.9kb, the use of common pAdeasy2 carrier can insert the 8.6kb. The specific use of the method can refer to the pAdeasy carrier system.
Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. We here report a strategy which simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, employing homologous recombination in bacteria rather than in eucaryotic cells. Following transfections of such plasmids into a mammalian packaging cell line, viral production is conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification.This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes.
pAdTrack-CMVprotocol.pdf（點擊下載）
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK722pAdTrack-CMV腺病毒質(zhì)粒.txt
