■ 基本信息
復制子: | pUC |
質粒分類: | 病毒系列，腺病毒載體 |
質粒大小: | 33477bp |
原核抗性: | Amp |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物: | M13F:TGTAAAACGACGGCCAGT |
3'測序引物: | 根據(jù)序列設計引物 |
■ 質粒屬性
質粒宿主: | 哺乳細胞,腺病毒 |
質粒用途: | 蛋白表達 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質粒簡介
The plasmid pAdEasy-1, containing most of the human adenovirus serotype 5 (Ad5) genome, is deleted for the genes E1 and E3. The removal of these two viral genes creates space for foreign DNA and eliminates selfreplication capabilities. The E1 deletion renders the viruses defective for replication and incapable of producing infectious viral particles in target cells (provided there is no complementation by the host cell); the E3 region encodes proteins involved in evading host immunity and is dispensable. The deletion of both genes creates room for up to 7.5 kb of foreign DNA that can be inserted into the Ad5 genome. The E1 gene, which is necessary for production of viral particles, is provided in trans by AD-293 cells. pAdEasy-1 carries the ampicillin resistance gene, which is lost after recombination with a shuttle vector.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK721pAdEasy-1腺病毒質粒.txt
