■ 基本信息
啟動子: | CMV |
復制子: | pUC |
終止子: | SV40 poly(A) signal，beta-globin polyA signal |
質(zhì)粒分類: | 哺乳細胞，四環(huán)素調(diào)控系統(tǒng)載體 |
質(zhì)粒大小: | 7139bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 四環(huán)素誘導 |
5'測序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'測序引物: | 根據(jù)序列設(shè)計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 蛋白表達 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質(zhì)粒簡介
pTet-On-Advanced expresses an improved version of the reverse Tet (tetracycline)-controlled transactivator protein (rtTA), called rtTA-Advanced (1–4). It is more sensitive to doxycycline (Dox) and yields lower background expression than the original rtTA used in the Tet-On? System (2). The rtTA-Advanced protein is a fusion of amino acids 1–207 of a mutant Tet repressor (TetR) and 39 amino acids containing three minimal "F"-type transcriptional activation domains from the VP16 protein of herpes simplex virus. It is fully synthetic, lacks cryptic splice sites, and is codon-optimized for stable expression in mammalian cells.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK693pTet-On-Advanced哺乳調(diào)控質(zhì)粒.txt
