■ 基本信息
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 哺乳細(xì)胞，信號通路報(bào)告載體 |
質(zhì)粒大小: | 4283bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測序引物: | 根據(jù)序列設(shè)計(jì)引物 |
3'測序引物: | 根據(jù)序列設(shè)計(jì)引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 信號報(bào)告 |
片段類型: | Promoter |
片段物種: | 空載體 |
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: | Fluc
|
■ 質(zhì)粒簡介
The pGL4.23[luc2/minP] Vector(a–e)encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4.23[luc2/minP] Vector contains a multiple cloning region for insertion of a response element of interest upstream of a minimal promoter and a luc2 gene. luc2 is a synthetically-derived luciferase sequence with humanized codon optimization. The vector backbone contains an ampicillin resistance gene to allow for selection in E. coli.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK477pGL4.23[luc2-minP]哺乳報(bào)告質(zhì)粒.txt
