■ 基本信息
別名: | pCDNA3-HA-N |
啟動子: | CMV |
復(fù)制子: | pUC |
終止子: | bGH poly(A) signal |
質(zhì)粒分類: | 哺乳細(xì)胞真核表達(dá)載體；蛋白過表達(dá)質(zhì)粒 |
質(zhì)粒大小: | 5484bp |
質(zhì)粒標(biāo)簽: | N-HA |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無需誘導(dǎo) |
5'測序引物: | T7：TAATACGACTCACTATAGGG |
3'測序引物: | SP6：ATTTAGGTGACACTATAGAA |
備注: |
載體上有起始密碼子ATG，注意不要移碼。 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | G418 |
熒光標(biāo)記: |
|
■ 質(zhì)粒簡介
pCDNA3.0-HA-N載體是專門設(shè)計用來在哺乳細(xì)胞中進(jìn)行高水平的穩(wěn)定表達(dá)和瞬時表達(dá)。其中人巨細(xì)胞病毒早期啟動子(CMV)在廣泛的哺乳動物細(xì)胞中可以實現(xiàn)高表達(dá)。新霉素抗性基因可以用來篩選穩(wěn)定細(xì)胞系，具體用G418篩選，另外HA做為標(biāo)簽?zāi)茉赪B實驗中更方便的檢測到目的基因是否表達(dá)。
pcDNA3.0-HA-N is 5.4 kb vectors designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
Neomycin resistance gene for selection of stable cell lines.
HA epitope for Western blot experiment.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK345pCDNA3-HA-N哺乳表達(dá)質(zhì)粒.txt
