■ 基本信息
啟動子: | T7 |
復(fù)制子: | ColA |
終止子: | T7 terminator |
質(zhì)粒分類: | 大腸桿菌載體；雙表達(dá)框質(zhì)粒 |
質(zhì)粒大小: | 3719bp |
質(zhì)粒標(biāo)簽: | N-6×His,C-S |
原核抗性: | Kan |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃，有氧，LB |
表達(dá)宿主: | BL21(DE3) |
誘導(dǎo)方式: | IPTG或乳糖及其類似物 |
5'測序引物: | ACYCDuetUP1：GGATCTCGACGCTCTCCCT DuetUP2:TTGTACACGGCCGCATAATC |
3'測序引物: | DuetDOWN1:GATTAGCGGCCGTGTACAA T7-ter:TGCTAGTTATTGCTCAGCGG |
備注: | 同時(shí)表達(dá)兩個(gè)外源蛋白 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 大腸桿菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 多框空載
|
原核抗性: | Kan |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡介
pCOLADuet-1 is designed for the coexpression of two target genes from a single plasmid. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). MCS-1 encodes the six-amino acid His?Tag sequence for the creation of a N-terminal fusion and MCS2 encodes the 15 amino acid S?Tag? peptide after the last restriction site for the creation of a C-terminal fusion if desired. Genes inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. Genes inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer. The vector has the COLA replicon from ColA(1) and the kanamycin resistance gene. This vector can be transformed into the same cell with plasmids containing compatible origins of replication and drug resistance genes for coexpression of up to 8 target genes.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK325pCOLADuet-1大腸雙表達(dá)質(zhì)粒.txt
