■ 基本信息
別名: | pET302NTHis |
啟動子: | T7 |
復制子: | pBR322 |
終止子: | T7 terminator |
質粒分類: | 大腸桿菌載體；PET系列表達質粒 |
質粒大小: | 5712bp |
質粒標簽: | N-6×His |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃，有氧，LB |
表達宿主: | BL21(DE3) |
誘導方式: | IPTG或乳糖及其類似物 |
5'測序引物: | T7:TAATACGACTCACTATAGGG |
3'測序引物: | T7-ter:TGCTAGTTATTGCTCAGCGG |
■ 質粒屬性
質粒宿主: | 大腸桿菌 |
質粒用途: | 蛋白表達 |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質粒簡介
The Champion pET302/NT-His and pET303/CT-His Vector Kit is designed for cloning a gene of interest via restriction enzyme(s) and ligase (REaL) and subsequent high-level expression from the strong bacteriophage T7 promoter. In addition to the T7 promoter, each vector contains only the necessary functional elements and an N- or C-terminal 6xHis tag (pET302/NT-His and pET303/CT-His, respectively) for convenient purification and detection (Figure 1). Expression levels obtained from these vectors may be higher than those obtained from another suppliers pET vector (Figure 2). To maximize expression, use with MagicMedia E. coli Expression Medium.
質粒只保證關鍵序列正確，不保證表達效果。
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK192pET302NT-His大腸表達質粒.txt
